Proliferasi Kalus dari Eksplan Hipokotil dan Kotiledon Tanaman Jarak Pagar (Jatropha curcas L.) pada Pemberian 2,4-D

Zulkarnain, Zulkarnain and Lizawati, Lizawati (2011) Proliferasi Kalus dari Eksplan Hipokotil dan Kotiledon Tanaman Jarak Pagar (Jatropha curcas L.) pada Pemberian 2,4-D. Jurnal Natur Indonesia, 14 (1). pp. 19-25. ISSN 1410-9379

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Abstract

The aim of this study was to develop an efficient method for the induction of embryogenic callus formation for in vitro propagation of jatropha. Plant materials used were 30-days old in vitro seedlings, cut into hypocotyl and cotyledon (lower, middle and upper) sections. Medium used was MS composition supplemented with vitamins, 3% sucrose, 0.7% agar at pH 5.8 ± 1, and 2,4-D (0, 1, 2, 3, 4 and 5 mg l-1). Cultures were kept at temperature of 25 ± 10C with 50 μmol m-2 s-1 light intensity and 16-h photoperiod. The results indicated that the rate of callus formation depended on the source of explant, the application of 2,4-D, and the interaction of both. The fastest callus proliferation (2.33 days following initiation) was obtained on cotyledon explants cultured on medium without 2,4-D. The explant sources and 2,4-D concentrations also showed significant effect on the percentage of explant forming callus. The most callus formation (88.33%) was obtained on middle cotyledon cultured on 3 mg l-1 2,4-D, whereas the fewest (6.84%) was found on upper cotyledon cultured on medium without 2,4-D. The colour of callus was dominated by white, light yellow, cream and brown with mostly compact structure, particularly on hypocotyl cultured on medium without 2,4-D. The texture of callus formed on hypocotyl treated with up to 4 mg l-1 2,4-D was dominated by coarse appearance. In contrast, majority of callus proliferated on hypocotyl treated with 5 mg l-1 2,4-D or cotyledon treated with or without 2,4-D produced callus with smooth texture.

Type: Article
Subjects: S Agriculture > SB Plant culture
Depositing User: ZULKARNAIN
Date Deposited: 27 Dec 2017 00:50
Last Modified: 27 Dec 2017 00:50
URI: http://repository.unja.ac.id/id/eprint/2591

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