Callus Proliferation from Immature Leaf Explants of Durian (Durio zibethinus Murr. cv. Selat) with the Addition of Picloram and BAP

Zulkarnain, Zulkarnain and Neliyati, Neliyati and Lizawati, Lizawati (2013) Callus Proliferation from Immature Leaf Explants of Durian (Durio zibethinus Murr. cv. Selat) with the Addition of Picloram and BAP. Jurnal Hortikultura Indonesia (Indonesian Journal of Horticulture), 4 (3). pp. 107-114. ISSN 2087-4855

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Abstract

This study was aimed to obtain an appropriate medium composition with various combinations of Picloram + BAP for the proliferation of embryogenic callus from immature leaf explants of durian. The experiment was carried out at the Plant Biotechnology Laboratory, Faculty of Agriculture, the University of Jambi from January through to November 2012. Five levels of Picloram (1.0, 2.0, 3.0, 4.0, 5.0 mg L-1) in combination with three levels of BAP (0, 0.5, 1.5 mg L-1) were tested. Therefore, there were 15 treatment combinations with 4 replicates resulting in 60 experimental unit. Each unit consisted of 4 culture flasks containing one immature leaf explant. Cultures were kept in culture room with 16 h photoperiod and 1000 lux light intensity. The results showed that: 1) callus proliferation on immature leaf explants of durian cv. Selat was dependent upon the level of Picloram + BAP added to culture medium, 2) the addition of 3.0 - 5.0 mg L-1 Picloram without BAP was found to be effective in promoting callus proliferation on the majority of cultured explants, 3) all regenerated callus showed similar characteristics, but embryogenic properties was not seen yet, and 4) the application of tissue culture technique in the propagation of durian cv. Selat needs further comprehensive investigation, particularly on factors directly affecting culture development and inducing somatic embryogenesis.

Type: Article
Subjects: S Agriculture > SB Plant culture
Depositing User: ZULKARNAIN
Date Deposited: 27 Dec 2017 00:50
Last Modified: 27 Dec 2017 00:50
URI: http://repository.unja.ac.id/id/eprint/2596

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