Extraction and Isolation Phytochemical and Antimicrobial Activity of Limonoid Compounds from Orange Waste Juice

Haroen, Ucop and Marlida, Yetti and Mirzah, Mirzah and Budianyah, Agus (2013) Extraction and Isolation Phytochemical and Antimicrobial Activity of Limonoid Compounds from Orange Waste Juice. Pakistan Journal of Nutrition, 12 (8). pp. 730-735. ISSN 1680-5194

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Official URL: http://scialert.net/abstract/?doi=pjn.2013.730.735

Abstract

Abstract: Antibacterial activity limonoid compounds of three different solvent extracts (methanol, ethyl acetate and n-heksane) prepared by soxhlet extractor from orange waste juice were screened against two pathogenic bacteria Escherichiacoli and Salmonella enteridis. The highest antibacterial potentiality was exhibited by the limonoid-ethyl acetate followed by the (limonoid-methanol) and (limonoid-n-hexane).The orange waste juice extract can be considered to be as equally potent as the antibiotics. The zone of inhibition, Minimum Inhibitory Concentration (MIC) of the orange waste juice extracts showed that limonoids extracted with ethyl acetate (limonoids-ethyl acetate) resulted in better inhibition of limonoids extracted with (limonoids-methanol) and (limonoids-n-hexane). The concentration 250 ppm of MIC were test showed inhibition of (limonoids-ethyl acetate) higher with a percentage minimum (44.15 for the bacterium Escherichia coli and 14.69 for the Salmonella enteridis) than (limonoids-methanol) (10.67 and 3.84) and (limonoids-n-hexane) (35.58 and 14.10). In vitro antibacterial screening of crude (limonoid-ethyl acetate) (limonoid-methanol) and (limonoid-n-hexane) of methanol extract of orange juice waste with concentration 250 ppm were test showed the clear zone in millimeters (11.75 for the bacterium Escherichia coli and 10.25 for the Salmonella enteridis) than limonoid-methanol (8.00 and 9.00) and limonoid-n-hexane (8.00 and 9.25). However, when compared to synthetic antibiotics (neomiditril and coleridin), the clear zone of limonoids lower (24%) but still showed his ability as an anti-microbial. (Limonoids-ethyl acetate) dose of 250 ppm showed better activity than doses of (limonoid-methanol) and (limonoid-n-heksana) for the bacterium Escherichia coli to Salmonella enteridis bacterial at 11.918 and 9.158. From these results we can conclude (limonoids-ethyl acetate) extract better than (limonoids-methanol) and (limonoids-n-hexane) and can be used as a substitute for anti- biotic synthetic antimicrobial. The phytochemical analysis of orange waste juice extracts showed presence of triter penes, alkaloid, flavonoids, steroid, phenolic, saponin.

Type: Article
Subjects: S Agriculture > SF Animal culture
Depositing User: UCOP HAROEN
Date Deposited: 06 Jun 2018 01:01
Last Modified: 06 Jun 2018 01:01
URI: http://repository.unja.ac.id/id/eprint/4357

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