Isolation and Amplification 16S rRNA gene for Metagenomic analysis from Oil Palm Rhizosphere in Different Locations (Based on Soil Textures)

Riany, Hesti and Mardiah, Ummi and Khairani, Khairani and Zulkarnain, Zulkarnain (2018) Isolation and Amplification 16S rRNA gene for Metagenomic analysis from Oil Palm Rhizosphere in Different Locations (Based on Soil Textures). In: International Symposium entitled “Socio-Ecological Transformations of Tropical Lowland Rainforests”, 7-12 October 2018, Bali, Indonesia.

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Abstract

The soil environment is the most complex habitat on earth. This complexity manages soil biodiversity, which is estimated to contain one third of all living organisms and regulates the activity of the organisms responsible for ecosystem functioning and evolution. It is known that most of them are soil bacteria. Soil microbes, especially bacteria, play key roles in ecosystems and influence a large number of important ecosystem processes, such as nutrient acquisition cycling, driving plant productivity and soil formation, biocontrol of some diseases, and fertilizer. Rhizosphere (root zone) is an important habitat for bacteria to play their function as plant growth promoter and plant protector from pathogen infection. Large area of oil palm tree plantation in Jambi Province will be good habitat for soil bacteria. Oil palm tree plantations in Jambi have different soil textures. There is an assumption that differrent soil texture lead to different diversity of soil bacteria in oil palm tree root zone. This research is started from collecting soil samples, followed by DNA extraction and amplification using 16S rRNA gene. This study aims to investigate the diversity of soil bacteria in oil palm rhizosphere from different soil textures. The study Result was obtained eight class of soil textures those were sandy loam, sandy clay, loamy sandy, salty clay, salty clay loam, sandy clay loam, sandy and clay. Genomic DNA purity were about 1,2-1,7. But 16S rRNA genes didnot obtain yet and need more optimation procedure to continue the study.

Type: Conference (Poster)
Subjects: Q Science > QR Microbiology
S Agriculture > S Agriculture (General)
S Agriculture > SD Forestry
Depositing User: ZULKARNAIN
Date Deposited: 07 Dec 2018 01:12
Last Modified: 07 Dec 2018 01:12
URI: http://repository.unja.ac.id/id/eprint/6245

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