Pollen viability and stigma receptivity in Sturt’s desert pea

Zulkarnain, Zulkarnain and Taji, Acram and Prakash, Nalamilli (2002) Pollen viability and stigma receptivity in Sturt’s desert pea. In: 12th Australasian Plant Breeding Conference, 15 - 20 September 2002, Hyatt Regency, Perth, Australia.

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Abstract

The work reported here was aimed at determining the duration of pollen viability and stigma receptivity in Swainsona formosa (Sturt’s desert pea, an Australian native legume), as an initial step in conventional breeding via artificial crossing by hand pollination. The pollen viability was determined for pollen shed from anthers and retained inside the keel under glasshouse conditions (temperature 25 - 32oC, light intensity ranging from 650 - 1200 µmol m-2 s-1 and photoperiod 10 – 14 hours) and for pollen harvested from anthers and stored under cold (4oC) and total darkness conditions. The receptivity of stigma was determined using stigma on intact plants under glasshouse conditions. The stigmatic surface was exposed and rubbed with fingertips. Pollen from anthers which had just begun shedding was applied to the stigmatic surface one day prior to anther dehiscence, on the day of anther dehiscence and daily thereafter until 8 days after anther dehiscence. The result indicated that pollen grains stored at 4oC remained fertile up to 21 days after storage (more than 50% of pollen germination was achieved on test solution). The pollen fertility in pollen grain retained inside the flower in the glasshouse declined within 5 days after shedding. The stigma receptivity reached a maximum 2 – 3 days after anther dehiscence and dropped significantly by day 6. The work reported here provides an insight into the correct timing for hand pollination in Sturt’s desert pea.

Type: Conference (Paper)
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: ZULKARNAIN
Date Deposited: 10 Dec 2018 01:17
Last Modified: 10 Dec 2018 01:17
URI: http://repository.unja.ac.id/id/eprint/6261

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