ANALISIS PLOIDI SECARA KONVENSIONAL DAN METODA FLOW CYTOMETRY

Zulkarnain, Zulkarnain (2004) ANALISIS PLOIDI SECARA KONVENSIONAL DAN METODA FLOW CYTOMETRY. Jurnal Ilmiah Universitas Batanghari Jambi, 4 (2). pp. 46-58. ISSN 1411-8939

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Abstract

Ploidy analysis, particularly the assessment on the chromosome number, of a species is a very crucial first step in plant breeding programme. Various ploidy analysis methods have been developed so far. The simplest method has been the staining of chromosomes in root tip or microspore cells. By this technique, the number of chromosomes can be determined precisely, and karyotype study of the chromosomes is likely to be conducted. Other method is by evaluating plant morphology or anatomical characteristics such as stomatal diameter and density, chlorophyll content within guard cells, diameter and viability of pollen grains, and leaf thickness, which are different according to the ploidy levels. Although this method offers a high accuracy, the staining technique or the evaluation of plant characteristics is not reliable to be used to evaluate the ploidy level of large scale population, because it is a time-consuming and laborious work. Now, a useful method for large-scale ploidy analysis, flow cytometry technique, has been developed. Although the initial investment is quite expensive, the benefit it offers for long-term large-scale ploidy analysis has made this method economically sound. This method of ploidy analysis rely on the nuclear DNA staining, which are then analyzed optically using an instrument called flow cytometer. The output of the reading is a histogram having different G1 peak for different ploidy levels. With the use of standard plant of known ploidy, the ploidy level of sample plant can be predicted based on the difference of their G1 peaks. This method is capable to analysis the ploidy level rapidly, but can not determine the number of chromosome. Both conventional and flow cytometry method have their own advantages and disadvantages. The choice of which method is going to be used will much depend upon the availability of resources and the objective of the works

Type: Article
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: ZULKARNAIN
Date Deposited: 17 Dec 2018 08:27
Last Modified: 17 Dec 2018 08:27
URI: http://repository.unja.ac.id/id/eprint/6415

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