THE EFFECT OF 2,4-D AND 2-iP ON CALLUS PROLIFERATION AND DEVELOPMENT ON IMMATURE LEAF EXPLANTS OF LIBERICA COFFEE (Coffea liberica L.)

Lizawati, Lizawati and Neliyati, Neliyati and Zulkarnain, Zulkarnain (2020) THE EFFECT OF 2,4-D AND 2-iP ON CALLUS PROLIFERATION AND DEVELOPMENT ON IMMATURE LEAF EXPLANTS OF LIBERICA COFFEE (Coffea liberica L.). Analele Universităţii din Oradea, Fascicula Biologie, 27 (1). pp. 39-42. ISSN 1844-7589

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Abstract

Abstract. An in vitro protocol for callus proliferation and development of Coffea liberica was developed. Immature leaf sections (1 x 1 cm) were cultured on solid MS medium supplemented with 2,4-D (0, 0.5, 1.0 and 2.0 mg L-1) and 2-iP (0, 1.0, 2.0 and 3.0 mg L-1). Cultures were maintained at temperature of 25 ± 1 °C, light intensity of 50 µmol m-2 s-1 and 16-hours daily photoperiod for 6 months. Callus growth and characteristics were evaluated for 6 months of culture. It was found that the application of 0.5 mg L-1 2.4-D + 2.0 mg L-1 2-iP resulted in the best time for callus induction (13.47 days after culture initiation). The application of 0.5 mg L-1 2.D + 2.0 mg L-1 2-iP and 1.0 mg L-1 2.4-D + 1.0 mg L-1 2-iP resulted in the highest frequency of explant forming callus (56.67% on the average). The morphology of proliferated callus varied from clear, white-creamy, light yellow and brown in color, and friable to compact in structures.

Type: Article
Subjects: S Agriculture > SB Plant culture
Depositing User: ZULKARNAIN
Date Deposited: 08 May 2020 15:20
Last Modified: 08 May 2020 15:20
URI: https://repository.unja.ac.id/id/eprint/10887

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