Identification of Mycobacterium Tuberculosis DNA in Polymerase Chain Reaction (PCR) Techniques in Formalin-Fixed Paraffin Embedded (FFPE) Extrapulmonary Tuberculosis

Fairuz, Fairuz and Dewi, Hasna (2019) Identification of Mycobacterium Tuberculosis DNA in Polymerase Chain Reaction (PCR) Techniques in Formalin-Fixed Paraffin Embedded (FFPE) Extrapulmonary Tuberculosis. Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI), Balik Papan. ISBN 978-602-60360-5-6

Text Proceeding book Annual Scientific Meeting 2019.pdf Download (1MB)

Abstract

Extrapulmonary tuberculosis (EPTB), which accounts for 10%–40% of the global burden of TB, is strongly associated with human immunodeficiency virus infection. Diagnosing EPTB is challenging, and recently, there has been a concerted effort to evaluate the latest molecular diagnostics for diagnosing TB in a range of specimen types. The Polymerase Chain Reaction (PCR) Techniquesis one such technology, which simultaneously detects Mycobacterium tuberculosis. Our objective was to evaluate the accuracy of the PCR for the diagnosis of EPTB in routinely processed formalin-fixed, paraffin-embedded (FFPE) tissues, compared with histological detection of TB as the gold standard. Methods: A convenience set of 68biobanked FFPE tissues from Hospital in Jambi Provence ( 2016-2018), including lymph nodes (n = 42), testis/scrotum (n = 3), abdominal tissue (n = 2), ovarium (n = 2), breast tissue (n =12), synovial tissue (n = 2), skin (n = 2), prostate (n = 2), and renal (n = 1), intestinal tract ( n= 4) from routine cases of clinically suspected EPTB were analyzed using the polymerase chain reaction (PCR) assay targeting IS6110, in parallel with Ziehl–Neelsen (ZN) staining, against histology as the gold standard. Results: Some 85% of specimens had histological evidence of TB infection. ZN staining was positive for TB in 12% of cases, and PCR was positive for TB in 62% of cases. Taking histology as the gold standard, the sensitivity and specificity were as follows: In lymph tissue the accuracy of the PCR was 41% (95%CI 37-67), not significantly better than ZN. In non-lymph tissue the sensitivity of the PCR was 82% (95%CI: 45%-95%). Conclusion: The PCR is potentially a useful tool for the diagnosis of TB in routine FFPE tissues especially for non-lymph tissue.

Type:	Book
Subjects:	R Medicine > RB Pathology
Divisions:	Fakultas Kedokteran dan Ilmu Kesehatan > Kedokteran
Depositing User:	Fairuz
Date Deposited:	02 Sep 2020 06:34
Last Modified:	02 Sep 2020 06:34
URI:	https://repository.unja.ac.id/id/eprint/13189

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