HIDAYAH, NURUL and Fairuz, Fairuz and Khatib, Alfi (2018) DEVELOPMENT OF INDUCED DIABETIC MODEL USING ZEBRAFISH. The 2nd Green Development International Conference (GDIC) . Universitas Jambi, Jambi.
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Abstract
Diabetes mellitus (DM) is a crucial metabolic disorder that had been increased over the years. The vast majority of diabetic population is suffered from type 2 diabetes mellitus (T2DM), followed by type 1 diabetes mellitus (T1DM). Although many anti-diabetic agents had been established, the incidence of diabetic complication is still rising. Then, further improvement on diabetic therapies is needed to be developed by using various animal model. In recent years, zebrafish is a newly found disease model organism in diabetes research as they have similar glucose metabolism pathways with mammals. Then, in the current study, we aimed to establish a protocol for inducing type 2 diabetes on zebrafish by using alternating immersion sugar solution method. Younger zebrafish (age 4-7 months) were divided into two group: glucose treated group and sucrose treated group with their respective control group. The fish are placed in the sugar solution for 24 hours, followed by free-sugar interval for the next 24 hours. The protocol had been modified by using stepwise increasing concentration of sugar solution for every 2 weeks. The experiment is continued for up to 8 weeks (2 months). The fasting blood glucose level and mortality rate of each treated group and control group are being determined at the end of experiment. The glucose treated group produces an increasing of fasting blood glucose level compared to control group. However, for sucrose treated group, the fasting blood glucose level is quite lower than control group which resulting in no diabetic effect on the fish. Overall, the mortality rate for both glucose and sucrose treated group are higher compared to their respective control group. In summary, the results do not show significant difference between treated group and control group. The method used need to be improvised according to the suitability condition of the zebrafish. As comparison with other research study that used similar protocol, they succeed to obtain desired outcome of hyperglycemic effect of zebrafish model.
| Type: | Book |
|---|---|
| Subjects: | Q Science > Q Science (General) R Medicine > RB Pathology |
| Depositing User: | Fairuz |
| Date Deposited: | 13 Nov 2020 02:15 |
| Last Modified: | 14 Oct 2021 01:33 |
| URI: | https://repository.unja.ac.id/id/eprint/14796 |
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