Disentangling the taxonomy of the subfamily Rasborinae (Cypriniformes, Danionidae) in Sundaland using DNA barcodes

Sholihah, Ami and Trotin, Erwan Delireu and Sukmono, Tedjo and Dahruddin, Hadi and Risdawaty, Renny and Elvyra, Roza and Wibowo, Arif and Kustiati, Kustiati and Busson, Frederic and Sauri, Sopian and Nurhaman, Ujang and Dounias, Edmon and Arifin, Syamsul and Fitriana, Yuli and Utama, Ilham and Muclisin, Zainal and Agnese, Jean and Hanner, Robert and Wowor, Daisy and Steinke, Dirk and Keith, Philippe and Ruber, Lukas and Hubert, Nicolas (2020) Disentangling the taxonomy of the subfamily Rasborinae (Cypriniformes, Danionidae) in Sundaland using DNA barcodes. Nature, 10 (2818). pp. 1-14. ISSN ISSN 1476-4687 (online)

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Abstract

Sundaland constitutes one of the largest and most threatened biodiversity hotspots; however, our understanding of its biodiversity is afflicted by knowledge gaps in taxonomy and distribution patterns. The subfamily Rasborinae is the most diversified group of freshwater fishes in Sundaland. Uncertainties in their taxonomy and systematics have constrained its use as a model in evolutionary studies. Here, we established a DNA barcode reference library of the Rasborinae in Sundaland to examine species boundaries and range distributions through DNA-based species delimitation methods. A checklist of the Rasborinae of Sundaland was compiled based on online catalogs and used to estimate the taxonomic coverage of the present study. We generated a total of 991 DNA barcodes from 189 sampling sites in Sundaland. Together with 106 previously published sequences, we subsequently assembled a reference library of 1097 sequences that covers 65 taxa, including 61 of the 79 known Rasborinae species of Sundaland. Our library indicates that Rasborinae species are defined by distinct molecular lineages that are captured by species delimitation methods. A large overlap between intraspecific and interspecific genetic distance is observed that can be explained by the large amounts of cryptic diversity as evidenced by the 166 Operational Taxonomic Units detected. Implications for the evolutionary dynamics of species diversification are discussed.

Type:	Article
Subjects:	Q Science > QL Zoology
Divisions:	Fakultas Sains dan Teknologi > Biologi
Depositing User:	Sukmono
Date Deposited:	24 Mar 2021 05:27
Last Modified:	24 Mar 2021 05:27
URI:	https://repository.unja.ac.id/id/eprint/17186

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