Phytochemical Screening and Quantitative Analysis of Coleus arthropurpureus Ethyl Acetate Fraction and Antibacterial Activity Against Staphylococcus aureus

Tarigan, Indra Lasmana Phytochemical Screening and Quantitative Analysis of Coleus arthropurpureus Ethyl Acetate Fraction and Antibacterial Activity Against Staphylococcus aureus. ALKIMIA: Jurnal Ilmu Kimia dan Terapan, 1 (4). pp. 17-23.

[img] Text
Pytochemical Screening.pdf
Download (313kB)

Abstract

Staphylococcus aureus (S. aureus) is a pathogenic microbe that is caused by various diseases in humans and animals. Infectious diseases caused by S. aureus in Asia reached 70% in 2007, while in Indonesia reached 23.5%. The plant provided several bioactive compounds that might function as an antibacterial which inhibits both bacterial growth and damaging the cell system and protein synthesis. Coleus arthropurpureus known contains alkaloids and tannins that supposed to be an antibacterial compound. Tannins have antibacterial activity, in general, the mechanism is to damage the bacterial cell membrane and induce the formation of complex compound bonds to enzymes or microbial substrates. This study aims to analyze the bioactive compounds contained in C. arthropurpureus in qualitative and quantitative which have an antimicrobial function using high-performance liquid chromatography (HPLC) in the reverse phase C-18 column and screening of antibacterial activity was carried out by disc diffusion method. The results of both qualitative and quantitative analysis by HPLC has obtained the presence of tannin bioactive compounds (1.48 ppm at a retention time of 2.806 minutes) and alkaloids (1.11 ppm at a retention time of 7.015). Moreover, we verified the diameter of inhibition of growth zone against S. aureus at a concentration of 15% extract was 12.80 mm. It was found that the highest percentage of the bioactive compound in C. arthropurpureus is tannin, and that is might an antibacterial agent.

Type: Article
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Sains dan Teknologi > Kimia
Depositing User: Indra Lasmana Tarigan
Date Deposited: 24 Mar 2021 01:57
Last Modified: 24 Mar 2021 01:57
URI: https://repository.unja.ac.id/id/eprint/17506

Actions (login required)

View Item View Item
Repository Unja is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.