EFFECT OF KETOGENIC DIET ON CELL PROLIFERATION IN THE LIVER OF WISTAR STRAIN WHITE RAT

Harahap, huntari and irfannuddin, irfannuddin and murti, krisna (2020) EFFECT OF KETOGENIC DIET ON CELL PROLIFERATION IN THE LIVER OF WISTAR STRAIN WHITE RAT. Unit Kerja dan Publikasi FKIK UNJA, Jambi. ISBN 978-602-73259-6-8

Text PROSIDING JAMHESIC diet ketogenic oke.pdf Download (272kB)

Abstract

Background: Ketogenic diet is one of the dietary management methods to overcome obesity. The ketogenic diet is a diet high in fat, sufficient protein, and low in carbohydrates. The main metabolic reactions due to the ketogenic diet occur in the liver. Hepatocytes, which are liver cells, can respond to oxidative stress due to the ketogenic diet in the form of cell proliferation. Liver cell proliferation can be determined by assessing the expression of Ki-67 Aim This study aimed to assess the accumulation of liver cells in Wistar rats after a ketogenic diet.. Method The method of this research is experimental laboratory research with a research design only post test group design. The study was conducted by examining body weight and blood ketones before and after the examination, then carrying out an immunohistochemical assessment of cell proliferation by assessing the expression of Ki-67. This study's inclusion criteria were male Wistar strain white rats (Rattus norvegicus), rats aged 2-3 months, body weight between 150-200 grams, and no anatomical abnormalities. Mice considered to drop out if the mice's blood ketone levels at week four were <0.5 and the mice died during the study period. Results of the study in this study were that there was an effect of differences in the diet intervention group on body weight after the diet intervention (p <0.05). There was an effect of differences in the dietary intervention group on blood ketone concentrations after the dietary intervention (p <0.05) without any impact on blood ketone concentrations before the dietary intervention. The average fraction of the expression area of Ki67 on the high-fat diet was 46.33%, the calorie restriction diet was 49.33%, the combination diet was 58.47%.In comparison, the percentage of the Ki-67 expression area fraction in control was 45.52%.. Conclusion A high-fat diet and a combination diet can significantly increase blood ketones compared to a calorie restriction and control diet. Diets high in fat, calorie restriction diets, and combination diets can increase liver cell proliferation. Keywords: Ketogenic Diet, Ki-67, Cell Proliferation.

Type:	Book
Subjects:	R Medicine > R Medicine (General)
Divisions:	Fakultas Kedokteran dan Ilmu Kesehatan > Kedokteran
Depositing User:	Harahap
Date Deposited:	22 Mar 2021 01:08
Last Modified:	22 Mar 2021 01:08
URI:	https://repository.unja.ac.id/id/eprint/18978

Actions (login required)

View Item