ASSESSING DISEASE ACTIVITY USING DISEASE ACTIVITY INDEX IN EXPERIMENTAL ANIMAL MODEL OF INFLAMMATORY BOWEL DISEASE INDUCED DEXTRAN

hanina, hanina and Tia Wida, Ekaputri and Rita, Halim and Lipinwati, Lipinwati (2020) ASSESSING DISEASE ACTIVITY USING DISEASE ACTIVITY INDEX IN EXPERIMENTAL ANIMAL MODEL OF INFLAMMATORY BOWEL DISEASE INDUCED DEXTRAN. The New Normal and Helath Science Practices, 1 (1). Jambi Medicine and Health Science International Conference, Virtual Conference. ISBN 978-602-73259-6-8

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Abstract

Background: Animal models of Inflammatory Bowel Disease (IBD) can be chemically induced using dextran sulfate sodium (DSS), trinitrobenzene sulfonic acid, and oxazolone. Mice showed different susceptibility and response to DSS. This study aimed to investigate the experimental animal model of ulcerative colitis induced by DSS using the Disease Activity Index assessment. Method: Nine male BALB/c mice with the weight of 25-40 grams were divided into 3 groups. Group I (control) was given drinking water, group II was given 2% DSS in drinking water for 5 days and exchanging DSS for water for 10 days, repeated 1 time, and Group III was given 3% DSS in drinking water for 7 days and exchanging DSS for water for 7 days, repeated 2 times. The Disease Activity Index score for group II and group III was zero. Result: The data of feed consumption for groups I, II, and III were 6 g/day, 6.5 g/day and 6.4 g/day, respectively. The data of drinking water consumption for groups I, II, and III were 5.8 ml/day, 6.3 ml/day and 6.2 ml/day, respectively. Administration of 2% DSS and 3% DSS did not showing clinical symptoms of ulcerative colitis in BALB/c mice. The study will be continued by analysing the colon samples of BALB/c mice macroscopically and microscopically.

Type:	Book
Subjects:	R Medicine > RZ Other systems of medicine
Divisions:	Fakultas Kedokteran dan Ilmu Kesehatan > Kedokteran
Depositing User:	Hanina
Date Deposited:	10 Jul 2024 03:45
Last Modified:	10 Jul 2024 03:45
URI:	https://repository.unja.ac.id/id/eprint/21552

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