Detection of mecA and mecC Staphylococcus aureus Isolates in Clinical Samples of Inpatients at Hospitals in Jambi in 2020

humaryanto, humaryanto and hanina, hanina and Fairuz, Fairuz (2020) Detection of mecA and mecC Staphylococcus aureus Isolates in Clinical Samples of Inpatients at Hospitals in Jambi in 2020. The new normal of medical care and education, 5 (-). Faculty of medicine Universitas Indonesia, online conference, jakarta. ISBN 978-989-758-433-6

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Abstract

MRSA is a strain of Staphylococcus aureus bacteria with phenotypic resistance characteristics to beta-lactam antibiotics, or low-affinity penicillin-binding protein (PBP) in B1subclass, and is often known as PBP2a or PBP2(3). PBP2a is characterized by the presence of mecA gene. Further research found MRSA isolates that did not contain the mecA gene. Sequencing analysis results on the isolate genome showed that the isolate carried the mecA homolog gene (mecALGA251) with an approximately 69% similarity with the classic mecA gene. This gene also encodes a protein with an approximately 63% similarity with PBP2a, and it was named the mecC gene in 2012. MRSA mecC is still rarely found in humans, but there have also been significant increasing cases in Denmark. Therefore, more extensive research on its prevalence in humans is needed. Until recently, no research has been conducted on the prevalence of MRSA mecC either in Jambi Province or in other regions in Indonesia. This study aims to determine the prevalence of MRSA mecC in a clinical sample of patients treated at hospitals in Jambi Province. This research was conducted at the Biomolecular Laboratory of the Faculty of Medicine and Health Sciences, Jambi University, from March to September 2020. Cultures were carried out on 117 clinical specimens in the forms of purulent wound swabs of the inpatients at hospitals in Jambi City. A total of 43 yellowish samples on the MSA agar were obtained, and sensitivity test was carried out against cefoxitin and Polymerase Chain Reaction (PCR) of mecA and mecC genes. Of the 43 S. aureus isolates, 20 were resistant to cefoxitin antibiotics. However, on PCR examination, 22 samples were found containing the mecA gene and none contained the mecC gene. A total of 51.16% of S. aureus isolates from clinical samples contained mecA gene, while mecC gene had not been found in S. aureus isolates in Jambi.

Type:	Book
Subjects:	R Medicine > RZ Other systems of medicine
Divisions:	Fakultas Kedokteran dan Ilmu Kesehatan > Kedokteran
Depositing User:	Hanina
Date Deposited:	10 Jul 2024 03:43
Last Modified:	10 Jul 2024 03:43
URI:	https://repository.unja.ac.id/id/eprint/21686

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