DETEKSI TITER ANTIBODI VIRUS AVIAN INFLUENZA (AI) MELALUI SWAB KLOAKA PADA TELUR AYAM BEREMBRIO (TAB) DI BALAI VETERINER (BVet) BUKITTINGGI

Arfandi, Ridho (2021) DETEKSI TITER ANTIBODI VIRUS AVIAN INFLUENZA (AI) MELALUI SWAB KLOAKA PADA TELUR AYAM BEREMBRIO (TAB) DI BALAI VETERINER (BVet) BUKITTINGGI. D3 thesis, Universitas Jambi.

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Official URL: https://repository.unja.ac.id/

Abstract

Dewasa ini peternak ayam sering risau akan adanya serangan Virus Avian Influenza (AI) atau sering dikenal dengan Flu Burung. Sehingga dibutuhkan deteksi titer antibodi ayam terhadap Virus AI yang mana diperlukan pengujian Serologi. Deteksi titer antibodi Virus Avian Influenza dilaksanakan di Laboratorium Virologi Balai Veteriner (Bvet) Bukittinggi.Deteksi titer antibodi Virus Avian Influenzadilaksanakan dengan menggunakan swab kloaka ayam yang di inokulasi pada media telur ayam berembrio (TAB) yang berumur 9-11 hari. Sampel yang di inokulasi pada TAB diinkubasi dengan suhu 35-37ºC dan dipanen cairan allantoisnya setelah 4-5 hari. Kemudian dilakukan pengujian Hemaglutinasi Assey (HA) setelah mendapatkan hasil pengujian HA maka dilanjutkan dengan pengujian Hemaglutinasi Inhibition (HI) diamana sampel diencerkan denganantigen 4 HA unit dan ditambahkan sel Darah Merah Ayam, kemudian diamkan di suhu kamar selama 50-60 menit. Sebanyak 293 sampel yang berasal dari 6 Kecamatan di Kabupaten Kampar, Provinsi Riau diperoleh hasil nagatif. Kemungkinan besar hal ini terjadi dikarenakan petugas setempat belum melaksanakan kegiatan vaksinasi terhadap Virus Avian Influenza. Avian Influenza Virus (AI) or often known as Bird Flu. So it is necessary to detect chicken antibody titers against AI Virus which requires serological testing. Detection of Avian Influenza Virus antibody titer was carried out at the Virology Laboratory of the Veterinary Center Bukittinggi. The detection of Avian Influenza Virus antibody titer was carried out using a chicken cloacal swab inoculated on 9-11 days old embryonic chicken egg media. Samples inoculated on embryonic chicken egg were incubated at a temperature of 35-37 and allantoic fluid was harvested after 4-5 days. Then the Hemaglutination Assey (HA) test was carried out after getting the HA test results, then continued with the Hemaglutination Inhibition (HI) test where the sample was diluted with 4 HA antigen units and added Chicken Red Blood cells, then let stand at room temperature for 50-60 minutes. A total of 293 samples from 6 sub-districts in Kampar Regency, Riau Province obtained negative results. Most likely this happened because local officials had not carried out vaccination activities against the Avian Influenza Virus.

Type: Thesis (D3)
Uncontrolled Keywords: Avian Influenza, Embryo Chicken Eggs, Antibody Titers
Subjects: L Education > L Education (General)
Divisions: Fakultas Peternakan > Kesehatan Hewan
Depositing User: RIDHO ARFANDI
Date Deposited: 23 Jul 2021 02:41
Last Modified: 23 Jul 2021 02:41
URI: https://repository.unja.ac.id/id/eprint/24080

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