Somatic (asexual) procedures (haploids, protoplasts, cell selection) and their applications

Tapingkae, Tanya and Zulkarnain, Zul and Kawaguchi, Masayo and Ikeda, Takashi and Taji, Acram (2012) Somatic (asexual) procedures (haploids, protoplasts, cell selection) and their applications. In: Plant Biotechnology and Agriculture: Propects for the 21st Century. Elsevier, Oxford, UK, pp. 141-162. ISBN 978-0-12-381466-1

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In angiosperms, double fertilization generates the embryo and the endosperm simultaneously, the joint development of which leads to a viable seed. Somatic embryos, however, are formed from plant cells that are not normally involved in the development of embryos (fertilization or gamete fusion). The term somatic refers to embryos developing asexually from vegetative (or somatic) tissue. Somatic plant cells are terminally differentiated and can regain totipotency and initiate embryo development under appropriate conditions. The development of techniques and protocols to produce plant embryos asexually has had a huge technological and economical impact on agricultural systems, and these biotechnologies represent an integral part of the breeding programs of important crops. In this chapter, recent advances in somatic (asexual) procedures and their applications are reviewed, including somatic embryogenesis, haploid technologies, protoplast and somatic hybridization, and use of somatic procedures in screening and development of stress-resistant plants. This method is now a well-established technology that has made significant contributions to plant improvement and mass propagation in horticulture, agriculture, and to some extent in forestry, as a means of rapidly multiplying elite varieties or clones.

Type: Book Section
Subjects: S Agriculture > SB Plant culture
Depositing User: Zulkarnain
Date Deposited: 27 Dec 2017 00:50
Last Modified: 27 Dec 2017 00:50

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