REV Tes similirity Identification SSCMec

humaryanto, humaryanto and hanina, hanina (2020) REV Tes similirity Identification SSCMec. IJTID, Malang.

Text 29% - Identification of SCC MEC Methicillin-Resistant Staphylococcus Aureus (MRSA) From Hospitals’ Clinical Samples in Jambi using Polymerase Chain Reaction (PCR).pdf Download (2MB)

Abstract

Staphylococcal cassette chromosome mec (SCCmec) is one of the mobile genetic elements of Methicillin-Resistant Staphylococcus aureus (MRSA) that carries many resistance genes and allows SCCmec to move from one bacterium to another. Twelve types of SCCmec have been identified throughout the world. Identification of SCCmec type is needed to determine the pattern of MRSA resistance in a particular region. This study aimed to identify the type of SCCmec MRSA from clinical samples. Specifically, this study was conducted at the Biomolecular Laboratory of the Faculty of Medicine and Health Sciences of Jambi University in June 2018-February 2019. Culture was carried out on 100 clinical specimens of festering wound swabs from inpatients at hopitals in Jambi City. A total of 32 samples of Staphytect plus test positive were tested using Cefoxitin disc diffusion method and MecA Polymerase Chain Reaction (PCR). There were 14 samples identified as MRSA isolates, namely twelve samples (85.72%) of SCCmec type III, one sample (7.14%) of SCCmec type II, and one sample (7.14%) of SCCmec type IVb. The results were different from previous studies where all MRSA isolates (100%) in Indonesia were SCCmec type III, although most SCCmec types were still dominated by SCCmec type III. This study concludes that there has been a shift in the content of SCCmec in MRSA isolate originating from hospitals in Jambi city. KEYWORDS MRSA; mecA; SCC mec, genetics, resistence

Type:	Other
Subjects:	A General Works > AC Collections. Series. Collected works Universitas Jambi (UNJA) > Fakultas Kedokteran dan Ilmu Kesehatan > A General Works > AC Collections. Series. Collected works
Divisions:	Library of Congress Subject Areas > A General Works > AC Collections. Series. Collected works Fakultas Kedokteran dan Ilmu Kesehatan > A General Works > AC Collections. Series. Collected works
Depositing User:	Humaryanto
Date Deposited:	22 Oct 2021 01:57
Last Modified:	22 Oct 2021 01:57
URI:	https://repository.unja.ac.id/id/eprint/27235

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