Safitri, Wulan (2022) ISOLASI SENYAWA METABOLIT SEKUNDER DARI EKSTRAK ETIL ASETAT DAUN SUNGKAI (Peronema canescens Jack) DAN UJI AKTIVITAS IMUNOMODULATOR. S1 thesis, Universitas Jambi.
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Abstract
Sungkai (Peronema canescens Jack.) or sabrang teak is one of the native plants in Indonesia which is traditionally used for dehumidifiers, treating malaria and maintaining health. People in Kerinci Regency use sungkai leaves to increase endurance. This indicates that the sungkai plant has chemical compounds that have the potential to be immunomodulatory compounds. Immunomodulators are substances that can change or affect the body's immune system towards normal. This study aims to isolate the compounds contained in the ethyl acetate extract of sungkai leaves and test the immunomodulatory activity of these compounds. Sungkai leaves are performed stratified maceration using n-hexane, ethyl acetate and methanol solvents. Furthermore, phytochemical tests were carried out to determine the compounds contained in ethyl acetate extract. Ethyl acetate extract contains flavonoid, phenolic and steroid compounds that have good immunomodulatory activity. The isolation process is continued using Liquid Vacuum Chromatography (KVC) so that isolates are obtained on F3. The KLT test using three different eluents showed one single stain. Phytochemical screening of F3 isolates contains steroid compounds. Immunomodulatory activity tests showed that at doses of 50 mg/Kgbb, 150 mg/Kgbb, 450 mg/Kgbb, K+ and isolates there was an increase in the number of leukocytes and % active macrophage activity than in the K-treatment. The treatment of extract doses of 50 mg/Kgbb, 150 mg/Kgbb and isolates of 0.7 mg/Kgbb was not significantly different from the K- treatment but was significantly different from the treatment of extract doses of 450 mg/Kgbb and K+. At a dose of 450 mg/Kgbb there was an increase in leukocytes and % of the highest active macrophage activity which was almost close to the K + treatment (Imboost 0.7 mg/Kgbb). The UV-Vis spectrum in the F3 isolate shows a maximum absorption peak at 267 nm indicating the presence of a nonconjugation double bond. The FTIR spectrum shows that the isolates have groups O-H (3381.13 cm-1), aliphatic C-H (2939.10 cm-1), C=C (1631.44 cm-1), C-H (1449.42-1362.82 cm-1), and C-O (1022.32 cm-1). The presence of the C=C and C-H groups indicates the presence of steroid group compounds. Based on a comparison of literature, the UV-Vis and FTIR spectrum patterns have similarities with the UV-Vis and FTIR spectra of the β-Sitosterol compounds. It is suspected that the compound resulting from the isolation of sungkai leaves is a steroid group compound, namely β-Sitosterol.
| Type: | Thesis (S1) |
|---|---|
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Sains dan Teknologi > Kimia |
| Depositing User: | safitri |
| Date Deposited: | 19 Jul 2022 07:01 |
| Last Modified: | 19 Jul 2022 07:01 |
| URI: | https://repository.unja.ac.id/id/eprint/37118 |
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