Triani, Anggi (2022) STUDI AKTIVITAS ANTIDIABETES SENYAWA BIOAKTIF DARI FRAKSI METANOL DAUN SUNGKAI (Peronema canescens Jack) SECARA IN VIVO DAN IN VITRO. S1 thesis, Jambi University.
![[img]](https://repository.unja.ac.id/style/images/fileicons/text.png) |
Text
SKRIPSI ANGGI AYUNDA TRIANI F1C118023.pdf Restricted to Repository staff only Download (1MB) | Request a copy |
|
Text
Cover.pdf Download (277kB) |
|
Text
Halaman Pengesahan.pdf Download (157kB) |
|
Text
ABSTRAK SKRIPSI ANGGI AYUNDA TRIANI.pdf Download (290kB) |
|
Text
BAB I SKRIPSI ANGGI AYUNDA TRIANI.pdf Download (299kB) |
|
Text
BAB V SKRIPSI ANGGI AYUNDA TRIANI.pdf Download (287kB) |
|
Text
DAFTAR PUSTAKA SKRIPSI ANGGI AYUNDA TRIANI.pdf Download (451kB) |
Abstract
Diabetes Mellitus is one of a group of metabolic diseases characterized by hyperglycemia caused by a disturbance in insulin so that blood glucose levels increase. Prevention of absorption of blood glucose by the intestine can be done with the help of the enzyme α-glucosidase. The secondary metabolite compounds of the flavonoid group have the effect of α-glucosidase inhibitors because they are able to regenerate the beta cells of the islets of Langerhans. Based on the results of phytochemical tests, it is known that the methanol fraction of sungkai leaves contains flavonoid compounds. The FTIR spectrum showed that the isolates had phenolic O-H groups (3,354.53 cm-1, 1,359.28 cm-1), aromatic C=C groups (1,615.48 cm-1), C-O-C eter groups (1,046.60 cm-1) and aromatic C-H group (822.21 cm-1). The presence of C=C and C-O-C groups indicates the presence of flavonoid compounds. Based on the comparison of the FTIR spectra which have similarities between the isolates and the literature, it is suspected that the methanol fraction of sungkai leaves contains flavonoid compounds. Antidiabetic activity tests were carried out in vivo and in vitro. In vivo antidiabetic activity test was carried out using test animals of white male mice which were induced orally by alloxan. Antidiabetic testing was carried out using 6 treatment groups with each group being repeated 3 times with K+ (glibenclamide), K- (Na-CMC 0.5%), K1 (methanol fraction of sungkai leaf dose 175 mg/kgBW), K2 (methanol fraction of sungkai leaf dose 350 mg/kgBW), K3 (methanol fraction of sungkai leaf dose 700 mg/kgBW) and isolates (isolate dose 2 mg/kgBW). Parameters observed were blood glucose levels before treatment, after induction and after treatment and body weight of mice. The antidiabetic activity of the methanol fraction of sungkai leaves was analyzed using statistical tests, namely blood glucose levels and body weight were tested one way ANOVA followed by Duncan's post hoc test. The results of the analysis showed that the methanol fraction of sungkai leaves had a significant effect on blood glucose levels in mice but had no significant effect on body weight of mice. The results showed that the methanol fraction of sungkai leaves had the best antidiabetic activity at a dose of isolate and a dose of 700 mg/kgBW which was able to reduce blood glucose levels by 42.20% and 42.00%, respectively, exceeding the effect of the antidiabetic drug glibenclamide. In vitro antidiabetic testing through -glucosidase enzyme inhibition mechanism did not show any antidiabetic activity at concentrations of fractions 5, 10, 25, 50 and 100 ppm. So it is suspected that the methanol fraction of sungkai leaves can reduce blood glucose levels through the mechanism of inhibition of the α-amylase enzyme.
| Type: | Thesis (S1) |
|---|---|
| Subjects: | Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Sains dan Teknologi > Kimia |
| Depositing User: | Triani |
| Date Deposited: | 20 Jul 2022 06:56 |
| Last Modified: | 20 Jul 2022 06:56 |
| URI: | https://repository.unja.ac.id/id/eprint/37832 |
Actions (login required)
 |
View Item |
