Polimorfisme Gen Hormon Pertumbuhan Pada Itik Kerinci Menggunakan Metode PCR-RFLP

Sukma Dewi, Ade (2022) Polimorfisme Gen Hormon Pertumbuhan Pada Itik Kerinci Menggunakan Metode PCR-RFLP. S1 thesis, UNIVERSITAS UNJA.

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Abstract

ABSTRAK. Tujuan penelitian ini untuk mengetahui bobot badan, Pertambahan Bobot Badan, dan ukuran tubuh itik Kerinci serta keragaman gen Growth Hormone. Materi penelitan yaitu 96 ekor itik Kerinci dan 96 sampel darah itik kerinci. Metode penelitian adalah eksperimen. Analisis data meliputi bobot badan, Pertambahan Bobot Badan dan ukuran tubuh itik Kerinci, asosiasi gen hormon pertumbuhan (GH), uji t, T2-hotelling, analisis komponen utama, frekuensi genotip dan alel, keseimbangan hardy-weinberg, heterozigositas, polymorphic information content (PIC). bobot badan, Pertambahan Bobot Badan, dan ukuran-ukuran tubuh itik Kerinci jantan berbeda nyata (P<0,05) lebih tinggi dibandingkan itik Kerinci betina. gen GH|XbaI mendapatkan tiga genotip yaitu ++ (41%), +- (33%), dan -- (25%)serta dua macam alel yaitu +(58%) dan –(41%). Populasi itik Kerinci berada dalam kesetimbangan Hardy-Weinberg. Heterozigositas populasi itik Kerinci berada dalam golongan sedang, nilai Ho(0,33)<He(0,48). PIC(0,42) primer yang digunakan cukup informatif sebagai penciri gen GH|XbaI. Gen GH|XbaI itik Kerinci berasosiasi dengan BB, PBB dan ukuran-ukuran tubuh. Kesimpulan: Rataan bobot badan, Pertambahan Bobot Badan, dan ukuran-ukuran tubuh itik Kerinci jantan lebih tinggi dibandingkan itik Kerinci betina. Penciri ukuran tubuh pada itik Kerinci jantan dan betina adalah panjang tulang dada, lingkar dada, dan panjang shank sedangkan penciri bentuk tubuh itik Kerinci jantan dan betina adalah panjang sayap. Gen GH|XbaI itik Kerinci bersifat polimorfik. genotip yang dinilai paling baik adalah genotip +/+. ABSTRACT. The goal of this study was to determine Kerinci duck body weight, weight gain, and body size, as well as the diversity of Growth Hormone genes. 96 Kerinci ducks and 96 Kerinci duck blood samples were used in the study. The method of investigation is experimental. Body weight, weight gain, and body size of Kerinci ducks were analyzed, as well as growth hormone (GH) gene association, t-test, T2-hotelling, principal component analysis, genotype and allele frequencies, hardy-weinberg balance, heterozygozity, and polymorphic information content ( PIC). Body weight, body weight gain, and body sizes of male Kerinci ducks were significantly different (P<0.05) higher than female Kerinci ducks. The GH|XbaI gene has three genotypes, namely ++ (41%), +- (33%), and -- (25%) and two alleles, namely +(58%) and –(41%). The Kerinci duck population is in Hardy-Weinberg equilibrium. The heterozygosity of the Kerinci duck population was in the medium group, the value Ho(0,33)<He(0,48). The PIC(0.42) primer used was quite informative as a marker of the GH|XbaI gene. The Kerinci duck GH|XbaI gene was linked to body weight, weight gain, and body measurements, with the ++ genotype performing the best. Male Kerinci ducks had higher average body weight, weight gain, and body sizes than female Kerinci ducks. Body size characteristics in male and female Kerinci ducks are sternum length, chest circumference, and shank length, while body shape characteristics in male and female Kerinci ducks are wing length. The GH|XbaI gene in Kerinci ducks is polymorphic. The best genotype is considered to be the +/+ genotype.

Type:	Thesis (S1)
Uncontrolled Keywords:	Kata kunci :Polimorfisme, gen Hormon Pertumbuhan, itik Kerinci, enzim XbaI
Subjects:	L Education > L Education (General)
Divisions:	Fakultas Peternakan > Peternakan
Depositing User:	DEWI
Date Deposited:	25 Jul 2022 04:16
Last Modified:	25 Jul 2022 04:16
URI:	https://repository.unja.ac.id/id/eprint/38120

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