Pengaruh Konsentrasi Zat Pengatur Tumbuh NAA dan Kinetin Terhadap Pertumbuhan Anggrek Dendrobium Woon Leng Secara Kultur Jaringan

Hasiana, Talita (2023) Pengaruh Konsentrasi Zat Pengatur Tumbuh NAA dan Kinetin Terhadap Pertumbuhan Anggrek Dendrobium Woon Leng Secara Kultur Jaringan. S1 thesis, Universitas Jambi.

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Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi zat pengatur tumbuh kinetin terhadap pertumbuhan anggrek Dendrobium Woon Leng secara kultur jaringan dan mengetahui konsentrasi manakah yang memberikan hasil terbaik terhadap pumbuhan anggrek Dendrobium Woon Leng secara kultur jaringan. Penelitian ini dilaksanakan di Laboratorium Bioteknologi Fakultas Pertanian Universitas Jambi. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan terdiri dari 2 faktor dengan 9 perlakuan yaitu : 1) Faktor 1 konsentrasi NAA terdiri dari 3 taraf ; (a) n1 = 0,5 ppm, (b) n2 = 1,0 ppm, (c) n3 = 1,5 ppm. 2) Faktor 2 konsentrasi kinetin terdiri dari 3 taraf ; (a) k1 = 1,0 ppm, (b) k2 = 3,0 ppm, (c) k3 = 3,5 ppm. Masing-masing jumlah perlakuan ada 9 setiap perlakuan diulang sebanyak 3 kali sehingga didapatkan sebanyak 27 satuan percobaan. Setiap satu unit percobaan terdiri dari 5 botol, jadi total keseluruhan yaitu 135 botol yang setiap botolnya diisi 1 eksplan. Setiap botol ditanam satu eksplan dengan ukuran eksplan yang digunakan pada ulangan I (1 cm), ulangan II (2 cm), ulangan III (3 cm). Dari hasil penelitian ini dapat diambil kesimpulan bahwa : 1) Pemberian konsentrasi NAA dan kinetin tidak terdapat interaksi terhadap variabel pengamatan jumlah daun, tinggi tanaman, dan panjang akar namun terdapat interaksi terhadap jumlah akar anggrek Dendrobium Woon leng. 2) Pemberian konsentrsi kinetin 1,0 ppm adalah konsentrasi terbaik untuk tinggi tanaman anggrek Dendrobium Woon leng. 3) Pemberian konsentrasi NAA lebih dari 1,5 ppm dapat menghasilkan jumlah akar dan panjang akar yang lebih banyak lagi untuk anggrek Dendrobium Woon leng. Kata kunci : Anggrek, Dendrobium Woon Leng, NAA dan Kinetin, kultur Jaringan   ABSTRACT This study aims to determine the effect of the concentration of kinetin growth regulator on the growth of Dendrobium Woon Leng orchids in tissue culture and to determine which concentration gives the best results on the growth of Dendrobium Woon Leng orchids in tissue culture. This research was conducted at the Laboratory of Biotechnology, Faculty of Agriculture, University of Jambi. This study used a randomized block design (RBD) consisting of 2 factors with 9 treatments, namely: 1) Factor 1 NAA concentration consisted of 3 levels; (a) n1 = 0.5 ppm, (b) n2 = 1.0 ppm, (c) n3 = 1.5 ppm. 2) Factor 2 kinetin concentration consists of 3 levels; (a) k1 = 1.0 ppm, (b) k2 = 3.0 ppm, (c) k3 = 3.5 ppm. Each treatment number was 9, each treatment was repeated 3 times so that a total of 27 experimental units were obtained. Each experimental unit consisted of 5 bottles, so the total was 135 bottles, each bottle filled with 1 explant. Each bottle was planted with one explant with the size of the explant used in replicate I (1 cm), replicate II (2 cm), replicate III (3 cm). From the results of this study it can be concluded that: 1) The concentration of NAA and kinetin did not have an interaction with the observed variables for the number of leaves, plant height, and root length, but there was an interaction with the number of roots of the Dendrobium Woon leng orchid. 2) Giving kinetin concentration of 1.0 ppm is the best concentration for Dendrobium Woon leng orchid plant height. 3) Giving NAA concentrations of more than 1.5 ppm can produce even more roots and root length for Dendrobium Woon leng orchids. Keywords: Orchid, Dendrobium Woon Leng, NAA and Kinetin, tissue culture

Type: Thesis (S1)
Uncontrolled Keywords: Anggrek, Dendrobium Woon Leng, NAA dan Kinetin, kultur Jaringan
Subjects: L Education > L Education (General)
Divisions: Fakultas Pertanian
Depositing User: SIBURIAN
Date Deposited: 07 Mar 2023 06:37
Last Modified: 10 Jul 2024 04:08
URI: https://repository.unja.ac.id/id/eprint/45374

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