Aktifitas Anti Bakteri dari Ekstrak Metanol Limbah Jus Jeruk (Citrus sinensis) Sebagai Feed Additive Alami (Antibacterial activity of the methanolic extract of orange (Citrus sinensis) juice waste as feed additive nature)

Haroen, Ucop (2015) Aktifitas Anti Bakteri dari Ekstrak Metanol Limbah Jus Jeruk (Citrus sinensis) Sebagai Feed Additive Alami (Antibacterial activity of the methanolic extract of orange (Citrus sinensis) juice waste as feed additive nature). Lembaga Penelitian Dan Pengabdian Kepada Masyarakat (LPPM) Universitas Jambi, Jambi.

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Abstract

ABSTRACT Antimicrobial effect of the crude organic extract of orange (Citrus sinensis) waste juice was studied in the Department of Agriculture, Faculty of Animal Husbandry Jambi University, during the period from May to June 2015. Disc diffusion and MIC method has been adopted in this study and petri dishes (120 mm in diameter) containing nutrient agar medium seeded with the test organism was used for antimicrobial screening. Test materials diffuse from the discs to the surrounding medium of the plate. The plates are then kept in an incubator (37ºC) for 18 hours to allow the growth of the microorganisms. The antibacterial activity of the test agent is determined by measuring the diameter of the zone of inhibition in term of millimeter. Antimicrobial screening showed that the crude methanol extract and other partially extracted fraction of the orange (Citrus sinensis) waste juice of possess antimicrobial activity against most of the test organisms depending upon the nature of their active ingredients in the extract and capacity of diffusion into the agar medium. Among the test organisms, the crude methanol extract of orange (Citrus sinensis) waste juice showed significant antimicrobial activity against Salmonella enteritidis, Esherichia coli. Keywords : Antibacterial activity, orange (Citrus sinensis), disc diffusion, MIC

Type: Other
Subjects: Q Science > Q Science (General)
Depositing User: Haroen
Date Deposited: 28 Jun 2018 01:02
Last Modified: 28 Jun 2018 01:02
URI: https://repository.unja.ac.id/id/eprint/4591

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