Haroen, Ucop and Syafwan, Syafwan and Kurniawan, Kiki and Budiansyah, Agus (2022) Determination of nutrient content, β-carotene, and antioxidant activity of Moringa oleifera extraction using organic solution. Network for veterinarians of Bangladesh, JOURNAL OF ADVANCED VETERINARY AND ANIMAL RESEARCH.
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Abstract
ABSTRACT Objective: The research was conducted to determine β-carotene and antioxidant activities and screening of phytochemical substances of Moringa oleifera extraction using organic solution. Materials and Methods: 550 gm of M. oliefera leaf flour was macerated. This research was con�ducted by laboratory experiments using the maceration method. The extraction was performed using three kinds of solvents, which are n-hexane, ethyl acetate, and methanol; for 3 x 24 h, they were concentrated with a rotary evaporator. Then, the flavonoid, phenolic, β-carotene isolation, and antioxidant tests were conducted using the 2,2-diphenyl-1-picrylhydrazyl on each fraction (n-hexane, ethyl acetate, and methanol). Results: The results of weighing each concentrated extract from the maceration process of each fraction (n-hexane, ethyl acetate, and methanol) were 12.67, 35.67, and 49.29 gm, with the total phenolic content (1.4595 ± 0.361, 46.5489 ± 1.832, and 39.74574 ± 0.786) and total flavonoid content of each fraction (3.3056 ± 0.039, 58.6389 ± 2.051, and 48.9056 ± 0.0809), respectively. The antioxidant activity test on the crude extract from the ethyl acetate fractionation showed that the IC50 value was 30.309 mg/ml. The ethyl acetate fraction has a high total phenolic and flavonoid content. The results of the isolation of β-carotene from M. oleifera leaf flour were 0.4798 gm, or equivalent to 0.956% carotenoids. Conclusions: Based on the results of the research, M. oleifera leaves are identified to have a fairly high antioxidant activity, which is 30.309 mg/ml, resulting from the potential compounds in M. oleifera leaves that function as inhibitors of antioxidant activity, which are the groups of phenolic and flavonoid compounds.
| Type: | Other |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Peternakan > Peternakan |
| Depositing User: | Budiansyah |
| Date Deposited: | 04 May 2023 07:03 |
| Last Modified: | 04 May 2023 07:03 |
| URI: | https://repository.unja.ac.id/id/eprint/47752 |
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