APLIKASI TEKNIK KULTUR JARINGAN PADA PERBANYAKAN TANAMAN JERUK NIPIS TANPA BIJI (Citrus aurantifolia)

Myrna E.F., Nyimas and Zulkarnain, Zulkarnain and Eliyanti, Eliyanti (1997) APLIKASI TEKNIK KULTUR JARINGAN PADA PERBANYAKAN TANAMAN JERUK NIPIS TANPA BIJI (Citrus aurantifolia). Buletin Agronomi Universitas Jambi, 1 (3). pp. 139-144. ISSN 1410-1939

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Abstract

This experiment was conducted at the Plant Tissue Culture Laboratory, Agricultural Faculty Unja, from September until December 1995. Three chemicals tested were Ca-hypochlorite, Na-hypochlorite and HgCl2 which were applied in 4 concentration levels, ie. 0.1, 0.5, 1.0 and 5.0%, and combined with 3 periods of explant dipping, ie. 10, 20 and 30 minutes. In addition to this, the media composition tested were MS, MS½, WPM, B-5, LS and SH. The result indicated that Ca- and Na-hypochlorite were not effective to be used in the sterilisation of single-node citrus explants taken from in vivo culture. Furthermore, even though there was a number of healthy explants, the treatment of HgCl2 concentration combined with dipping period showed a non-significant effect on the culture growth. In addition, there was also no significant effect resulted in six media composition tested.

Type: Article
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: ZULKARNAIN
Date Deposited: 10 Dec 2018 01:17
Last Modified: 10 Dec 2018 01:17
URI: https://repository.unja.ac.id/id/eprint/6252

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