Zulkarnain, Zulkarnain and Smith, Jennifer and Taji, Acram (2005) PROLINE: A BIOCHEMICAL INDICATOR FOR THE EMBRYOGENIC POTENTIAL OF SWAINSONA FORMOSA CALLUS. In: Conference of the Australian Branch of the International Association for Plant Tissue Culture and Biotechnology, 21 - 24 September 2005, Perth, Australia.

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An experiment was conducted to determine the association between endogenous proline and the embryogenic potential of callus mass proliferated from the anther wall tissue of Swainsonia formosa. Callus proliferation was initiated from anthers containing microspores at early- to late-uninucleate stages pre-treated with water or mannitol starvation at 4 oC for 2 days, and cultured on a double-phase B5 medium supplemented with vitamins, 2% sucrose and 49.3 μM IBA and 4.61 μM zeatin. Ficoll-400 was added at 5 to 20% (w/v) concentrations to enhance embryogenic callus formation. After 4 weeks in total darkness, cultures were placed under cool fluorescent light with an intensity of 50 μmol m-2 s-1 for a 16 hour photoperiod at 25  1 oC. Somatic embryos were successfully regenerated from embryogenic callus. Non-embryogenic callus continued to grow, and resulted in further callus proliferation when transferred to a regeneration medium supplemented with 1% sucrose and 4.63 μM kinetin. Microscopic observation combined with amino acid analysis of callus tissue using ethanol extraction and sulphosalicylic acid provided strong evidence that embryogenic potential was correlated with endogenous proline content; embryogenic callus contained proline whereas proline was absent in non-embryogenic callus. This suggests that proline quantification may provide an effective biochemical method to assess the embryogenic, and/or proline my be used to improve embryogenic potential of callus mass.

Type: Conference (Paper)
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: Zulkarnain
Date Deposited: 10 Dec 2018 01:17
Last Modified: 10 Dec 2018 01:17

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