In vitro callus induction and differentiation on Sturt’s Desert Pea (Swainsona formosa)

Zulkarnain, Zulkarnain and Taji, Acram and Prakash, Nalamilli (2002) In vitro callus induction and differentiation on Sturt’s Desert Pea (Swainsona formosa). In: 12th Australasian Plant Breeding Conference, 15 - 20 September 2002, Hyatt Regency, Perth, Australia.

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Callus growth was induced on media containing IAA (0.57, 5.71, 57.1 µM and IBA (0.49, 4.93, 49.3 µM were combined with BA (0.44, 4.44, 44.4 µM), kinetin (0.46, 4.63, 46.3 µM), 2iP (0.49, 4.93, 49.3 µM) and zeatin (0.46, 4.61, 46.1 µM). The result indicated that callus formation was significantly affected by IAA + BA, IBA + BA, IBA + 2iP, IBA + kinetin and IBA + zeatin. Among IAA combinations, IAA at 5.71 µM or 57.1 µM in combination with 44.4 µM BA produced the highest callus formation (26%). Meanwhile, with the use of IBA the highest callus formation (38%) was obtained on 49.3 µM IBA + 0.44 µM BA, followed by 36% on 4.49 µM IBA + 44.4 µM 2iP, 4.93 µM IBA + 4.63 µM kinetin and 0.49 µM IBA + 4.61 µM zeatin, respectively. The texture and colour of callus varied widely from being compact to friable and white translucent to dark green in colour depending on the types of plant hormones used. However, green embryogenic callus was formed on media supplemented with IBA + kinetin and IBA + zeatin and, subcultured onto a new medium with similar hormones or onto a hormone-free medium. After two weeks in culture, callus grown on the hormone-free medium showed no further growth, turned chlorotic and died. Meanwhile, callus subcultured onto a medium containing IBA + kinetin produced agglomerates of green small shoots without root or roots without shoot and, callus grown on a medium supplemented with IBA + zeatin showed only further callus growth. Some of these shoots and roots, however, were found to be abnormal in appearance. Shoots were hyperhydrated, necrotic or chlorotic, and eventually died after 16 weeks in culture. Some of the roots were short and thick with no root hairs, and grew directly from within the callus.

Type: Conference (Paper)
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: Zulkarnain
Date Deposited: 10 Dec 2018 01:17
Last Modified: 10 Dec 2018 01:17

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