Zulkarnain, Zulkarnain and Taji, Acram and Prakash, Nalamilli (2001) BREEDING STRATEGIES USED IN PRODUCTION OF STERILE STURT'S DESERT PEA (SWAINSONA FORMOSA) FLOWERS. In: The 20th International EUCARPIA Symposium, Section Ornamentals: Strategies for New Ornamentals, Melle, Belgium.

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One of the impediments to commercialisation of Sturt's Desert Pea a cut flower is the production of large amount of pollen grain in flowers. This brings about the reduction in flower quality due to petal staining by the pollen grains which are shed during transportation. In addition, during transportation pollination may occur, resulting in rapid degeneration of flowers and thus reduction in the vase life of the flowers. Our work is focussed on the production of sterile plants by manipulating the ploidy level. The strategies used are via in vitro and in vivo techniques. In vitro strategy is centred around the production of haploid plants using anther culture. Since pollen grains are haploid, plants regenerated from them are also haploid. With the in vivo strategy, we aim to produce triploid plants. Step one in the process is to produce plants that are tetraploid by application of chemical colchicine (colchicine acts by disrupting spindle formation during mitotic cell division) to immature floral apices at the right developmental stage. These flowers, upon pollination and fertilisation, will produce seeds that are tetraploid. Crossing of diploid and tetraploid plants will result in production of triploid plants. Both haploid and triploid plants are sterile because where there is an odd number of chromosome sets reproductive fertility is usually impaired. This is because during meiosis the normal pairing of chromosome can not properly take place since one set of chromosomes will have no homologous set to pair with, and as such gametes fail to form.

Type: Conference (Poster)
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: Zulkarnain
Date Deposited: 10 Dec 2018 01:18
Last Modified: 10 Dec 2018 01:18

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