PENGARUH SUHU DAN LAMA PRA-PERLAKUAN MANITOL TERHADAP PROLIFERASI KALUS PADA KULTUR ANTERA Swainsona formosa

Zulkarnain, Zulkarnain (2005) PENGARUH SUHU DAN LAMA PRA-PERLAKUAN MANITOL TERHADAP PROLIFERASI KALUS PADA KULTUR ANTERA Swainsona formosa. Jurnal Tropika, 13 (1). pp. 52-56. ISSN 0854-6533

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Abstract

The aim of this investigation was to study the effect of anther stress pre-treatment using mannitol at two different temperature regimes and four different periods of time on the growth of anthers cultured in vitro. Anthers with microspores at uninucleate stage were pre-treated with mannitol at 4oC or 25oC for 2, 4, 6 or 8 days in dark condition. Following this, the anthers were cultured on solid B5 medium supplemented with vitamins and 2% sucrose at light intensity of 50 µmol m-2 s-1 and 16 hours photoperiod at 25 ± 1oC. The results indicated that stress pre-treatment enhanced callus formation from cultured anthers. Pre-treatment of anthers with mannitol starvation at 4oC for 2 - 8 days produced more callus than mannitol starvation at 25oC for the same period. Although this pre-treatment might not sufficient to induce microspore embryogenesis in S. formosa, it was clear that anther pre-treatment with mannitol starvation at 4oC for 8 days showed the capacity of promoting the in vitro tissue growth.

Type: Article
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: Zulkarnain
Date Deposited: 17 Dec 2018 03:18
Last Modified: 17 Dec 2018 03:18
URI: https://repository.unja.ac.id/id/eprint/6401

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