Zulkarnain, Zulkarnain (2004) Pengaruh Ficoll dan Pra-perlakuan Stres terhadap Embriogenesis Somatik pada Kultur Antera Swainsona formosa. Hayati, 11 (3). pp. 121-124. ISSN 0854-8587
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Abstract
One of the impediments to the commercialisation of Sturt’s desert pea (Swainsona formosa) as a cut flower is the release of large amounts of pollen grains during anther dehiscence. These pollen grains may stain the petals and so reduce the quality of the flowers. In addition, self-pollination can occur during transportation causing flowers to degenerate quickly. This project was undertaken with the objective to investigate strategies to overcome such problems through an in vitro approach by anther culture. The objective of the project was to induce the regeneration of pollenless haploid plants. Of the variables tested, pre-treating the anthers with mannitol starvation at 4oC followed by introduction to a double-phase medium supplemented with Ficoll-400 were found to enhance callus proliferation. Embryogenic callus was produced and embryogenesis was detected but these embryos did not originate from microspores. Instead, the embryos grew from sporophytic tissue of the anther wall. The embryos failed to develop further when subcultured to root and shoot induction medium due to a high frequency of hyperhydration. Morphogenesis was detected but shoots and roots developed poorly.
| Type: | Article |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture |
| Depositing User: | Zulkarnain |
| Date Deposited: | 17 Dec 2018 08:27 |
| Last Modified: | 17 Dec 2018 08:27 |
| URI: | https://repository.unja.ac.id/id/eprint/6417 |
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