OPTIMIZATION OF THE GROWTH REGULATOR CONCENTRATION FOR THE INDUCTION OF SOMATIC EMBRYOGENESIS OF LIBERICA COFFEE (Coffea liberica L. cv. Liberica Tungkal Composite)

Lizawati, Lizawati and Kartika, Elis and Antony, Dedy and Purnamaningsih, Ragapadmi (2024) OPTIMIZATION OF THE GROWTH REGULATOR CONCENTRATION FOR THE INDUCTION OF SOMATIC EMBRYOGENESIS OF LIBERICA COFFEE (Coffea liberica L. cv. Liberica Tungkal Composite). Analele Universităţii din Oradea, Fascicula Biologie, 31 (1). pp. 7-14. ISSN 1844-7589

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Abstract

The liberica coffee (Coffea liberica L. cv. Liberica Tungkal Composite) is a type of coffee that has been widely developed in the lowland areas of Jambi Province, Indonesia specifically in West Tanjung Jabung and East Tanjung Jabung Regencies. However, the mother tree of liberica coffee is old and attacked by many diseases, so that a fast seed propagation technique, tissue culture is an efficient plant propagation technique. This study aims to examine the interaction of auxin and cytokinin concentrations that are most optimal for the induction of somatic embryogenesis of liberica coffee. The experiment used a completely randomized design factorial pattern with 2 factors, the first factor was auxin 2,4-D and the second factor was cytokinin 2-iP treatment. The results revealed that application of different dosage of growth regulators 2,4-D and 2-iP in the forming of somatic embryogenesis of liberica coffee showed an interaction with the observational variables of span time for callus proliferation, percentage of explant forming callus, and callus size. The balanced application of growth regulators 2,4-D and 2-iP was able to induce the formation of somatic embryogenesis from liberica coffee immature leaf explants. The most optimal interaction for the development of embryo stages from globular, heart and torpedo is 5 μM 2,4-D + 5 μM 2-iP treatment.

Type:	Article
Subjects:	S Agriculture > SB Plant culture
Divisions:	Fakultas Pertanian > Agroekoteknologi
Depositing User:	Lizawati
Date Deposited:	04 Jun 2024 00:47
Last Modified:	04 Jun 2024 00:47
URI:	https://repository.unja.ac.id/id/eprint/64296

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