EVALUASI PROFIL GAS BERBAGAI PAKAN BERSERAT MENGGUNAKAN TEKNIK IN-VITRO

Mardhiah, Nur Aysah (2024) EVALUASI PROFIL GAS BERBAGAI PAKAN BERSERAT MENGGUNAKAN TEKNIK IN-VITRO. S1 thesis, Universitas Jambi.

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Abstract

Abstrak Latar Belakang: Pakan sumber serat dapat berasal dari limbah pertanian dan agroindustri seperti jerami padi, oil palm decanter meal dan ampas tebu. Ketersediaan pakan ini cukup melimpah dan berpotensi dimanfaatkan sebagai pakan. Pemanfaatan ini perlu dievaluasi profil gasnya menggunakan teknik in-vitro. Tujuan: Mendapatkan hasil evaluasi profil gas yang terbaik meliputi produksi gas kumulatif, potensi produksi gas (b), laju produksi gas (c), gas metana (CH4) dan pH rumen dari pakan berserat seperti limbah pertanian dan agroindustri dengan menggunakan teknik in vitro. Metode: Persiapaan sampel bahan yang digunakan seperti rumput kumpai (P0), jerami padi (P1), oil palm decanter meal (P2) dan ampas tebu (P3). Persiapan pelaksanaan metode in-vitro meliputi persiapan tabung fermentor, persiapan inokulum rumen, pembuatan anaerobik medium dan pelaksanaan inkubasi selama 48 jam. Pengukuran gas kumulatif dan metana yang dilakukan pada lama inkubasi 2, 4, 6, 8, 10, 12, 24 dan 48 jam. Pengukuran pH yang dilakukan diakhir inkubasi lalu berikan HgCl2 jenuh untuk menghentikan aktivitas mikroba dalam tabung fermentor. Hasil: Berdasarkan hasil penelitian didapatkan masing-masing perlakuan berpengaruh nyata (P<0.05) terhadap produksi gas kumulatif, potensi produksi gas (b), laju produksi gas (c), produksi gas metana dan pH rumen. Kesimpulan: Penelitian ini dapat disimpulkan bahwa setiap perlakuan memiliki produksi gas yang bervariasi namun nilai produksi gas kumulatif, produksi gas metana dan potensi produksi gas (b) tertinggi dimiliki oleh P0 dan diikuti oleh P3. Laju produksi gas (c) tertinggi dimiliki oleh P3. pH rumen yang tertinggi dimiliki oleh P2. Sehingga profil gas yang memiliki nilai terbaik adalah P3. Abstract Background: Feed sources of fiber can come from agricultural and agroindustrial waste such as rice straw, oil palm decanter meal and bagasse. The availability of this feed is quite abundant and has the potential to be used as feed. This utilization needs to be evaluated for its gas profile using in-vitro techniques. Objective: To obtain the best gas profile evaluation results including cumulative gas production, gas production potential (b), gas production rate (c), methane gas (CH4) and rumen pH from fibrous feed such as agricultural and agroindustrial waste using in vitro techniques. Methods: Preparation of samples of materials used such as kumpai grass (P0), rice straw (P1), oil palm decanter meal (P2) and bagasse (P3). Preparation for the implementation of the in-vitro method includes preparation of fermenter tubes, preparation of rumen inoculums, manufacture of anaerobic medium and incubation for 48 hours. Cumulative gas and methane measurements were made at incubation periods of 2, 4, 6, 8, 10, 12, 24 and 48 hours. pH measurements are taken at the end of incubation and then saturated HgCl2 is applied to stop microbial activity in the fermenter tube. Results: Based on the results of the study, each treatment had a real effect (P<0.05) on cumulative gas production, gas production potential (b), gas production rate (c), methane gas production and rumen pH. Conclusion: This study can be concluded that each treatment has a variable gas production but the highest cumulative gas production value, methane gas production and gas production potential (b) are owned by P0 and followed by P3. The highest gas production rate (c) is owned by P3. The highest rumen pH is owned by P2. So that the gas profile that has the best value is P3.

Type:	Thesis (S1)
Uncontrolled Keywords:	in-vitro; kinetik degradasi; limbah berserat; metana; profil gas.
Subjects:	L Education > L Education (General)
Divisions:	Fakultas Peternakan > Peternakan
Depositing User:	MARDHIAH
Date Deposited:	19 Jul 2024 07:03
Last Modified:	19 Jul 2024 07:03
URI:	https://repository.unja.ac.id/id/eprint/69113

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