Karakterisasi Keragaman Gen Prolaktin dan Produksi Telur Hasil Persilangan Ayam Merawang dan Ayam Arab (MA)

Raysa, Regina (2025) Karakterisasi Keragaman Gen Prolaktin dan Produksi Telur Hasil Persilangan Ayam Merawang dan Ayam Arab (MA). S1 thesis, Universitas Jambi.

	Text COVER.pdf Download (86kB)
	Text ABSTRAK.pdf Download (125kB)
	Text HALAMAN PENGESAHAN.pdf Download (200kB)
	Text BAB I.pdf Download (167kB)
	Text BAB V.pdf Download (154kB)
	Text DAFTAR PUSTAKA.pdf Download (183kB)
	Text FULL SKRIPSI.pdf Restricted to Repository staff only Download (2MB)

Abstract

Penelitian ini bertujuan untuk mengetahui karakterisasi keragaman gen prolaktin dengan produksi telur hasil persilangan ayam Merawang dengan ayam Arab (MA). Materi yang digunakan pada penelitian ini yaitu 66 ekor ayam MA, terdiri dari 30 ekor jantan dan 36 ekor betina yang dipelihara selama 5 bulan. Data yang dihimpun meliputi bobot badan, pertambahan bobot badan, dan produksi telur serta sampel darah. Identifikasi keragaman gen PRL dilakukan dengan analisis PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) dengan enzim pemotong Mnl1. Analisis data yang digunakan meliputi uji beda rata-rata (Uji-t) antar bobot badan ayam MA dengan tetuanya. Analisis data molekuler meliputi: frekuensi genotip dan alel, keseimbangan Hardy-Weinberg, Heterozigositas, Polymorphic Information Content (PIC), dan asosiasi gen prolaktin dengan bobot badan dan produksi telur ayam MA. Hasil penelitian ini menunjukkan bahwa rataan bobot badan dan pertambahan bobot badan ayam MA lebih tinggi jika dibandingkan ayam Arab namun lebih rendah jika dibandingkan ayam Merawang. Keragaman gen PRL|Mnl1 ayam MA betina bersifat polimorfik dengan genotip +/+ (0,14), +/- (0,36) dan -/- (0,50). Hasil analisis uji chi-square menunjukkan bahwa X2 hitung sebesar 1,03 berada dalam keseimbangan Hardy- Weinberg, nilai Heterozigositas ayam MA betina sebesar 0,44. Nilai PIC ayam MA sebesar 0,39. Gen PRL memiliki asosiasi yang signifikan (P<0.05) dengan bobot badan dan produksi telur ayam MA. Ayam MA dengan genotipe +/+ memiliki bobot badan yang lebih tinggi, sedangkan ayam MA dengan genotipe -/- memiliki produksi telur yang lebih tinggi dibandingkan ayam MA genotipe +/+ dan +/-. Kesimpulan: keragaman gen prolaktin pada produksi telur bersifat polimorfik, Gen PRL memiliki asosiasi yang signifikan (P<0.05) dengan bobot badan genotipe +/+ dan produksi telur pada genotipe -/-. This research aims to characterize the diversity of prolactin genes with egg production from the crossing of Merawang chickens with Arab chickens (MA). The material used in this study was 66 MA chickens, consisting of 30 males and 36 females reared for 5 months. Data collected included body weight, body weight gain, and egg production as well as blood samples. Identification of PRL gene diversity was carried out by PCR-RFLP analysis (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) analysis with the Mnl1 cutting enzyme. Data analysis used included mean difference test (t-test) between body weight of MA chickens and their parents. Molecular data analysis included: genotype and allele frequencies, Hardy-Weinberg equilibrium, Heterozygosity, Polymorphic Information Content (PIC), and association of prolactin gene with body weight and egg production of MA chickens. The results showed that the average body weight and body weight gain of MA chickens were higher than those of Arab chickens but lower than those of Merawang chickens. The diversity of the PRL|Mnl1 gene in female MA chickens is polymorphic with genotypes +/+ (0.14), +/- (0.36) and -/- (0.50). The results of the chi-square test analysis showed that X2 count of 1.03 was in Hardy-Weinberg balance, the Heterozygosity value of female MA chicken is 0.44. The PIC value of MA chicken is 0.39. The PRL gene had a significant association (P<0.05) with body weight and egg production of MA chickens. MA chickens with +/+ genotype had higher body weight, while MA chickens with -/- genotype had higher egg production than +/+ and +/- genotype MA chickens. Conclusion: prolactin gene diversity in egg production is polymorphic, PRL gene has significant association (P<0.05) with body weight of +/+ genotype and egg production in -/- genotype.

Type:	Thesis (S1)
Uncontrolled Keywords:	Ayam MA, Produksi Telur, Gen Prolaktin (PRL), PCR- RFLP.
Subjects:	L Education > L Education (General) S Agriculture > SF Animal culture
Divisions:	Fakultas Peternakan > Peternakan
Depositing User:	RAYSA
Date Deposited:	12 Jun 2025 03:35
Last Modified:	12 Jun 2025 03:36
URI:	https://repository.unja.ac.id/id/eprint/79569

Actions (login required)

View Item