In Vitro Callus Formation from Male Inflorescences of Oil Palm (Elaeis guineensis Jacq.) by The Application of Picloram

Kartika, Elis and Lizawati, Lizawati and Zulkarnain, Zulkarnain (2019) In Vitro Callus Formation from Male Inflorescences of Oil Palm (Elaeis guineensis Jacq.) by The Application of Picloram. Analele Universităţii din Oradea, Fascicula Biologie, 26 (1). pp. 57-61. ISSN 1844-7589

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Abstract

This study aimed at studying the effect of different concentrations of Picloram on callus proliferation from young male inflorescences explants of oil palm. The inflorescences were isolated from 5 - 6 years old stock plants grown in the field. The concentration of Picloram tested were 10, 20, 30, 40, 50, 60, 70 and 80 ppm. Each treatment was repeated 3 times, and each replication consisted of 3 explants in one culture flask. Culture medium used was solid MS composition supplemented with 0.03% activated charcoal. Cultures were kept in light conditions at 27 ± 1 °C for 16 weeks without subculture. Observations were performed on the percentage of explants forming callus, time span to first callus proliferation, and the characteristics of callus (color and structure). Results showed that Picloram could increase the percentage of explants forming callus, where Picloram at 50 ppm was able to produce the highest percentage of callusing explants (77.78% on the average). In addition, the 50 ppm Picloram was also able to accelerate callus proliferation to an average of 10.23 days after culture initiation. The proliferated callus showed uniform characteristics, ie. cream, yellowish cream and brownish cream in colour, with a friable structure indicating embryogenic characteristics.

Type: Article
Subjects: S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Depositing User: Zulkarnain
Date Deposited: 12 Jun 2019 03:38
Last Modified: 12 Jun 2019 03:38
URI: https://repository.unja.ac.id/id/eprint/8036

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