Preservasi Ovarium dan Pengaruhnya Terhadap Morfologi Folikel Domba

Rosadi, Bayu and Setiadi, M Agus and Sajuthi, Dondin and Boediono, Arief (2011) Preservasi Ovarium dan Pengaruhnya Terhadap Morfologi Folikel Domba. Jurnal Veteriner, 12 (2). pp. 91-97. ISSN 1411 - 8327

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The purpose of this study was to evaluate the effect of cooling and freezing of ewes ovarian tissue on their follicles morphology. The study was carried out in two experiments. Experiment I, ovaries were maintained in Phosphate Buffered Saline (PBS) at -20o C and room temperature (RT) for 24 h, and at 5o C for 24 h and 72 h, respectively. After storage, follicles were histologically evaluated. Experiment II, the ovarian cortex was isolated and tissue slices (±1 mm3) were prepared. Following this tissues were loaded into hemistraw then transferred to equilibration solution (PBS+20% FBS+7,5% EG+7,5 % DMSO) at room temperature and held for 10, 20, 30 minutes, respectively. Afterward tissues were tranferred to vitrification solution (PBS+20%FBS +15%EG+15%DMSO ) for 3 minutes, then the hemistraw was placed directly into liquid nitrogen. After thawing, the tissues were prepared for histological examination. All of the follicles were deteriorated after 24 h storage at RT. The percentage of morphologically normal follicles was significantly reduced when ovarian tissues were stored at -20o C for 24 h and at 5o C for 24 h and 72 h. However, it seemed to have a minor deterioration effect when the tissues were kept at 5o C for 24 h (P<0.05). Antral follicles were damaged in all of the treatments. Primordial follicles preserved their morphology intactness better than growing follicles. Exposing tissues to equilibration medium for 10 minutes seemed to produce higher numbers of morphologically normal follicles (P<0.05), compared to when tissues were exposed for 20 minutes and 30 minutes (P>0.05). It can be concluded that exposing tissues to equilibration solution for 10 minutes prior to freezing would kept the ovarian follicles morphology in good condition

Type: Article
Subjects: S Agriculture > SF Animal culture
Divisions: Fakultas Kedokteran dan Ilmu Kesehatan > Ilmu Keperawatan
Depositing User: Rosadi
Date Deposited: 24 Sep 2019 01:51
Last Modified: 24 Sep 2019 01:51

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