PENGARUH PENAMBAHAN MOLASSES PADA INOKULUM FESES PADA TEKNIK IN VITRO : UJI AMONIA, pH, DAN TOTAL PROTOZOA

MANDALA PUTRA, MUHAMMAD UTAMA (2024) PENGARUH PENAMBAHAN MOLASSES PADA INOKULUM FESES PADA TEKNIK IN VITRO : UJI AMONIA, pH, DAN TOTAL PROTOZOA. S1 thesis, UNIVERSITAS JAMBI.

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Abstract

In vitro merupakan metoda yang sering digunakan untuk mengevaluasi degradasi suatu bahan pakan dengan menggunakan inokulum cairan rumen yang biasanya diperoleh dari sapi berfistula. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan molasses ke dalam cairan feses terhadap pH, konsentrasi amonia, dan jumlah populasi protozoa. Materi yang digunakan pada penelitian adalah tepung rumput gajah, cairan rumen, inokulum cairan feses, larutan McDougall, gas CO2, termos, plastik, kain kasa, termometer, inkubator, tabung fermentor, watherbath, clumper, declumper, glasshiring, tabung gas CO2 dan sentrifuge. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 4 ulangan dengan perlakuan RL (Cairan rumen/kontrol), RF (Cairan feses/kontrol), RF2 (Cairan feses dan 2 % molases), RF3 (Cairan feses dan 3 % molases) dan RF4 (Cairan feses dan 4 % molases). Peubah yang diamati adalah pH, konsentrasi amonia dan total protozoa. Data dianalisis menggunakan sidik ragam dan bila berpengaruh nyata, maka dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa penambahan molasses dalam inokulum feses berpengaruh sangat nyata (P<0,01) terhadap pH rumen, dimana semakin tinggi konsentrasi penambahan molasses dalam inokulum feses menyebabkan pH rumen semakin menurun. Hasil uji Duncan menunjukkan bahwa konsentrasi pH pada perlakuan RL berbeda sangat nyata (P<0,01) dengan RF, RF2, RF3 dan RF4. Selanjutnya perlakuan RF menunjukkan hasil yang tidak berbeda nyata (P>0,01) dengan RF2, tetapi berbeda sangat nyata (P<0,01) dengan RF 3 dan RF4. Selanjutnya penambahan molasses dalam inokulum feses berbasis sampel rumput gajah berpengaruh sangat nyata (P<0,01) terhadap kadar ammonia rumen, dimana hasil uji DMRT menunjukkan bahwa RF3 tidak berbeda nyata (P>0,01) dengan RF4, tetapi berbeda sangat nyata (P<0,01) dengan RF, RL dan RF. Akan tetapi, penambahan molasses dalam inokulum feses berpengaruh tidak nyata (P>0,01) terhadap total protozoa rumen. Kesimpulan dari penelitian ini penambahan molasses sebanyak 2% ke dalam cairan feses mempengaruhi nilai pH, amonia dan total protozoa. Penambahan molasses 2% dalam feses mendekati nilai pH, amonia dan total protozoa cairan rumen secara in vitro. In vitro is a method that is often used to evaluate the degradation of a feed ingredient using rumen fluid inoculum which is usually obtained from cows with fistulas. This research aims to determine the effect of adding molasses to feces on pH, ammonia concentration and protozoan population numbers. The materials used in the research were elephant grass flour, rumen fluid, fecal fluid inoculum, McDougall's solution, CO2 gas, thermos, plastic, gauze, thermometer, incubator, fermenter tube, watherbath, clumper, delumper, glasshiring, CO2 gas tube and centrifuge . This study used a completely randomized design with 5 treatments and 4 replications with treatments RL (rumen fluid/control), RF (fecal fluid/control), RF2 (fecal fluid and 2% molasses), RF3 (fecal fluid and 3% molasses) and RF4 (Fecal fluid and 4% molasses). The variables observed were pH, ammonia concentration and total protozoa. The data was analyzed using variance and if the effect was significant, it was continued with the Duncan test. The results showed that the addition of molasses to the feces inoculum had a very significant effect (P<0.01) on rumen pH, where the higher the concentration of molasses added to the feces inoculum caused the rumen pH to decrease. Duncan's test results showed that the pH concentration in the RL treatment was very significantly different (P<0.01) from RF, RF2, RF3 and RF4. Furthermore, RF treatment showed results that were not significantly different (P>0.01) from RF2, but very significantly different (P<0.01) from RF 3 and RF4. Furthermore, the addition of molasses in the faecal inoculum based on elephant grass samples had a very significant effect (P<0.01) on rumen ammonia levels, where the DMRT test results showed that RF3 was not significantly different (P>0.01) from RF4, but was very significantly different ( P<0.01) with RF, RL and RF. However, the addition of molasses in the fecal inoculum had no significant effect (P>0.01) on total rumen protozoa. The conclusion from this study was that adding 2% molasses to feces affected the pH value, ammonia and total protozoa. The addition of 2% molasses in feces approaches the pH, ammonia and total protozoa values ​​of rumen fluid in vitro.

Type:	Thesis (S1)
Uncontrolled Keywords:	Molasses, Inokulum Feses, In Vitro, Uji Amonia, pH, Protozoa
Subjects:	L Education > L Education (General)
Divisions:	Fakultas Peternakan > Peternakan
Depositing User:	PUTRA
Date Deposited:	17 Jul 2024 08:28
Last Modified:	17 Jul 2024 08:28
URI:	https://repository.unja.ac.id/id/eprint/68507

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